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Saccharomyces cerevisiae
Also known as: Saccharomyces, S. cerevisiae
Facts (61)
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Adaptive laboratory evolution – principles and applications for ... link.springer.com Jul 1, 2013 59 facts
claimOverflow metabolism in Saccharomyces cerevisiae may have evolved to provide an evolutionary advantage in specific environments and to protect carbon resources from competing species.
claimAlmario MP, Reyes LH, and Kao KC demonstrated that Saccharomyces cerevisiae can be evolutionarily engineered to exhibit enhanced tolerance to lignocellulosic biomass hydrolysates.
referenceJ. Wright, E. Bellissimi, E. De Hulster, A. Wagner, J.T. Pronk, and A.J. Van Maris compared batch and continuous culture-based selection strategies for acetic acid tolerance in xylose-fermenting Saccharomyces cerevisiae in a 2011 study published in FEMS Yeast Research.
claimSaccharomyces cerevisiae can evolve anticipatory gene regulatory patterns under cycling salt and oxidative stress conditions within 300 generations.
claimThe evolutionary potential of Saccharomyces cerevisiae strains is suggested to be limited by polyploidy, which provides increased genetic robustness compared to haploid laboratory strains.
measurementIn typical E. coli or S. cerevisiae cultures, a fitness increase of 50–100% can be achieved within 100 to 500 generations, which corresponds to approximately 2 months of selection.
claimVan Maris and co-workers evolved a S. cerevisiae strain with deficient pyruvate decarboxylase activity for high-glucose tolerance, which was later attributed to an in-frame deletion in the glucose regulator MTH1 that altered the stability of the MTH1-encoded protein.
measurementSelection for improved galactose utilization in S. cerevisiae via serial transfer resulted in a 24% increase of maximum growth rate (μmax) within 62 days, along with increased galactose uptake rates and ethanol production.
claimSaccharomyces cerevisiae exhibits adaptive cross-protection, where cobalt resistance improves tolerance to other metal ions, and pulsed cobalt stress exposure during laboratory evolution results in cross-protection to thermal and oxidative stress.
referenceK.C. Kao and Gavin Sherlock characterized clonal interference during adaptive evolution in asexual populations of Saccharomyces cerevisiae in a 2008 study published in Nature Genetics.
claimIncreased ethanol tolerance in Saccharomyces cerevisiae is mediated by mutations in the translational regulator SSD1 and UTH1, indicating that cell wall stability is a major factor in ethanol tolerance.
claimAvrahami-Moyal L et al. used turbidostat culture to apply selective pressure of ethanol on Saccharomyces cerevisiae W303-1A, which selected for mutations in the SSD1 and UTH1 genes.
claimMutations in RAS/PKA signaling pathways serve as a source for increased fitness in Saccharomyces cerevisiae when grown on galactose medium.
claimGlucose-limited growth in S. cerevisiae led to the emergence of aneuploidy and the amplification of the HXT6 gene, with significant divergence observed between co-evolved populations.
claimIn the last 25 years, there has been an increasing number of adaptive laboratory evolution experiments, with Escherichia coli and Saccharomyces cerevisiae being the most prominent organisms under investigation.
claimAerobic chemostat-selected Saccharomyces cerevisiae cells showed increased fitness in anaerobic glucose-limited chemostats and acetate-limited chemostats, but decreased fitness in nitrogen-limited batch cultivations.
referenceOud et al. (2012) published 'An internal deletion in MTH1 enables growth on glucose of pyruvate-decarboxylase negative, non-fermentative Saccharomyces cerevisiae' in Microbial Cell Factories, volume 11, page 131.
claimDhar R et al. studied the adaptation of Saccharomyces cerevisiae to saline stress through laboratory evolution.
claimIn the model yeast Saccharomyces cerevisiae, adaptation towards salt tolerance leads to increased cell size and increased ploidy.
claimIncreased copper tolerance in Saccharomyces cerevisiae is mediated by a genomic amplification of CUP1, decreased basal levels of the copper transporters CTR2 and CCC2, and decreased activity of antioxidant enzymes.
referenceWisselink et al. (2010) analyzed the metabolome, transcriptome, and metabolic flux of arabinose fermentation by engineered Saccharomyces cerevisiae.
referenceJansen et al. (2005) found that prolonged selection in aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae causes a partial loss of glycolytic capacity, published in Microbiology.
claimMesophilic organisms such as Escherichia coli and Saccharomyces cerevisiae have inherent properties that limit their use in high-temperature processes, necessitating the use of non-conventional microbial species in biotechnology.
claimBatch selection for freeze-thaw tolerance in Saccharomyces cerevisiae also selects for increased thermal, oxidative, and ethanol stress resistance.
referenceZhou et al. (2012) enabled rapid xylose utilization and ethanol production by Saccharomyces cerevisiae through xylose isomerase overexpression, engineering of the pentose phosphate pathway, and evolutionary engineering.
claimFrequent mutations in HXT genes and glucose sensors and regulators, such as RGT1 and MIG2, were identified in S. cerevisiae during glucose-limited chemostat growth.
referenceWisselink et al. (2009) developed an evolutionary engineering approach for the accelerated utilization of glucose, xylose, and arabinose mixtures by engineered Saccharomyces cerevisiae strains.
referenceShen et al. (2012) obtained an efficient xylose-fermenting recombinant Saccharomyces cerevisiae strain through adaptive evolution and analyzed its global transcription profile.
claimGlucose-limited selection in S. cerevisiae chemostats led to increased biomass yield and decreased fermentative capacity.
claimAdaptation towards salt tolerance in Saccharomyces cerevisiae involves gene expression changes in CTT1 and MSN4, as well as a high-frequency SNP in the transcriptional regulator MOT2.
referenceGarcia Sanchez et al. (2010) used evolutionary engineering to improve xylose and arabinose utilization by an industrial recombinant Saccharomyces cerevisiae strain.
referenceZelle et al. (2011) established an anaplerotic role for cytosolic malic enzyme in engineered Saccharomyces cerevisiae strains.
claimCadière A et al. developed evolutionary engineered Saccharomyces cerevisiae wine yeast strains that exhibit increased in vivo flux through the pentose phosphate pathway.
claimIncreased tolerance of Saccharomyces cerevisiae towards lignocellulosic hydrolysates, such as acetic acid and furfural, is correlated with adaptive changes of the oxidative stress response.
claimCross-stress protection in Saccharomyces cerevisiae is asymmetrical, where oxidative stress protects against salt stress, but salt stress does not protect against oxidative stress.
referenceDe Kok et al. (2012) identified new lactate transporter genes in a jen1Δ mutant of Saccharomyces cerevisiae as ADY2 alleles using laboratory evolution, whole-genome resequencing, and transcriptome analysis.
claimGeneral evolutionary principles observed in Escherichia coli and Saccharomyces cerevisiae, such as the tendency toward optimized biomass yield, overflow metabolism, large-scale regulatory changes, and the emergence of mutator strains, also occur in other microbial hosts like Lactococcus lactis.
referenceZelle et al. (2010) identified phosphoenolpyruvate carboxykinase as the sole anaplerotic enzyme in Saccharomyces cerevisiae.
referenceCakar, Turanli-Yildiz, Alkim, and Yilmaz (2012) studied the evolutionary engineering of Saccharomyces cerevisiae to improve industrially important properties in FEMS Yeast Research.
measurementApproximately 30% of genes in well-studied organisms such as Escherichia coli and Saccharomyces cerevisiae have unknown functions.
claimCakar ZP et al. utilized evolutionary engineering to develop strains of Saccharomyces cerevisiae that are resistant to multiple stresses.
referencePaquin and Adams (1983) reported that relative fitness can decrease in evolving asexual populations of Saccharomyces cerevisiae in the journal Nature.
claimIncreased gene copy numbers of heterologous genes in recombinant Saccharomyces cerevisiae can emerge during evolutionary engineering and contribute to increased growth in anaerobic conditions.
claimResearchers have improved the carbon source utilization of Saccharomyces cerevisiae strains by combining genetic engineering with evolutionary adaptation.
claimSaccharomyces cerevisiae mutants can evolve cross-protection towards lignocellulosic hydrolysates, showing increased fitness with inhibitor combinations but reduced fitness when exposed to individual hydrolysate components.
referenceWisselink et al. (2007) engineered Saccharomyces cerevisiae for efficient anaerobic alcoholic fermentation of L-arabinose, published in Applied and Environmental Microbiology.
referenceVan Maris et al. (2004) achieved directed evolution of pyruvate decarboxylase-negative Saccharomyces cerevisiae, resulting in a C2-independent, glucose-tolerant, and pyruvate-hyperproducing yeast strain, published in Applied and Environmental Microbiology.
claimResearchers used ethylmethanesulfonate (EMS)-treated Saccharomyces cerevisiae to achieve increased nutrient utilization and stress resistance.
claimCakar ZP et al. isolated cobalt hyper-resistant mutants of Saccharomyces cerevisiae using an in vivo evolutionary engineering approach.
referenceKuyper et al. (2005) performed evolutionary engineering of mixed-sugar utilization by a xylose-fermenting Saccharomyces cerevisiae strain, published in FEMS Yeast Research.
claimZelle and co-workers established growth on glucose in a pyruvate carboxylase-negative S. cerevisiae strain by identifying a point mutation in the recombinant sfcA gene, which shifted the co-factor preference from NADH to NADPH.
measurementEvolutionary rescue frequency is positively correlated with stress concentration in Saccharomyces cerevisiae but negatively correlated in Saccharomyces paradoxus over 100 generations of evolution.
referenceDunham et al. (2002) identified characteristic genome rearrangements in the experimental evolution of Saccharomyces cerevisiae, published in the Proceedings of the National Academy of Sciences USA.
referenceSonderegger and Sauer (2003) performed evolutionary engineering of Saccharomyces cerevisiae to enable anaerobic growth on xylose, published in Applied and Environmental Microbiology.
referenceJansen et al. (2004) demonstrated that prolonged maltose-limited cultivation of Saccharomyces cerevisiae selects for cells with improved maltose affinity and hypersensitivity, published in Applied and Environmental Microbiology.
referenceScalcinati et al. (2012) applied evolutionary engineering to Saccharomyces cerevisiae to achieve efficient aerobic xylose consumption.
referenceAdamo GM, Lotti M, Tamás MJ, and Brocca S published 'Amplification of the CUP1 gene is associated with evolution of copper tolerance in Saccharomyces cerevisiae' in Microbiology in 2012.
claimGlucose-limited growth in S. cerevisiae resulted in decreased activity levels of glycolysis-related enzymes, correlating changes in the expression levels of genes ENO1, ENO2, TDH1, and PYK1, and down-regulation of the stress response genes MSN2 and MSN4.
claimSulfate limitation in Saccharomyces cerevisiae leads to mutations affecting TOR signaling via an RRN3 mutation and genomic amplification of the high-affinity sulfate transporter SUL1.
A critical review of industrial fiber hemp anatomy, agronomic ... bioresources.cnr.ncsu.edu 1 fact
claimEdible-coated packaging made of gelatin with hempseed oil applied to golden apples, cheese, and pork demonstrated antibacterial activity against Penicillium expansum, Saccharomyces cerevisiae, Staphylococcus aureus, and Escherichia coli pathogens.
Medicinal plants: bioactive compounds, biological activities ... frontiersin.org 1 fact
claimThe alkaloid tomatidine, found in potatoes, inhibits enzymes involved in ergosterol production in many species of Candida and Saccharomyces.