Knowledge Tree
partial reprogramming
Also known as: reversible reprogramming, in vivo partial reprogramming, partial cell reprogramming, partial reprogramming, partial reprogramming of the epigenome
Facts (14)
Sources
Cellular rejuvenation: molecular mechanisms and potential ... - Nature nature.com Mar 14, 2023 13 facts
referenceBrowder et al. (2016) published 'In vivo amelioration of age-associated hallmarks by partial reprogramming' in Cell, demonstrating that partial reprogramming can reduce aging signs in living organisms.
claimA non-integrative partial reprogramming protocol using a cocktail of mRNAs carrying OSKM + LIN28 alleviates cellular aging in naturally aged human fibroblasts and endothelial cells by resetting the epigenetic clock, reducing inflammatory responses in chondrocytes, and restoring youthful regenerative responses without altering cellular identity.
claimOSKM-mediated partial reprogramming restores youthful gene expression in adipocytes, mesenchymal stem cells (MSCs), and myogenic cells, while single-cell genomics reveals that this process temporarily suppresses somatic identity programs.
claimLong-term partial reprogramming in physiologically aging mice rejuvenates tissues such as the skin and kidneys and the organism as a whole, characterized by a reversion of the epigenetic clock and metabolic and transcriptomic changes, including decreased expression of genes mediating inflammation, senescence, and stress response pathways.
referenceLehmann et al. discuss partial reprogramming as an emerging strategy for safe induced cell generation and rejuvenation.
referenceOcampo et al. (2022) published 'Diverse partial reprogramming strategies restore youthful gene expression and transiently suppress cell identity' in Cell Systems, comparing different partial reprogramming methods.
procedureCellular reprogramming for cardiac regenerative medicine treats cardiovascular disease (CVD) through three methods: (1) in vitro reprogramming of cells like fibroblasts into cardiomyocytes by initiating cardiac development programs to provide a cell source for regeneration; (2) in vivo cardiac reprogramming using lineage transcription factors or microRNA manipulation to convert resident cardiac fibroblasts into cardiomyocytes in situ; (3) partial reprogramming of mature cardiomyocytes into a fetal state to restore function in senescent cells by temporarily reactivating nascent developmental programs.
referenceAlderton (2014) discusses the risk that partial reprogramming induces cancer in Nature Reviews Cancer.
claimMendelsohn, Larrick, and Lei (2019) assert that partial reprogramming of the epigenome can induce cellular rejuvenation.
claimPartial reprogramming is a tool for the restoration of the youthful state of cells.
referenceA partial reprogramming strategy for intervertebral disc rejuvenation by activating an energy switch was described by Ribeiro et al. in Aging Cell (2022).
claimPartial reprogramming can reduce the epigenetic age of cells without causing a loss of cell identity, suggesting that full reprogramming into induced pluripotent stem cells (iPSCs) is not required to reverse the aging of somatic cells.
claimOSKM + LIN28-mediated partial reprogramming in senescent human fibroblasts results in the recovery of high mobility of histone protein 1β, which is a feature characteristic of young fibroblasts.
Toward precision longevity: aging interventions in the single-cell ... frontiersin.org Dec 4, 2025 1 fact
referenceBrowder et al. (2022) demonstrated that in vivo partial reprogramming alters age-associated molecular changes during physiological aging in mice, published in Nature Aging.