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related 3.58 — strongly supporting 11 facts
Mice and nonhuman primates are both utilized as model organisms in the same experimental protocol to test neural theories of consciousness, as evidenced by their shared roles in behavioral tasks [1], cross-species comparisons [2], and specific neural manipulation procedures {fact:1, fact:5, fact:6}.
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Protocol for testing global neuronal workspace and integrated ... journals.plos.org 11 facts
procedureThe researchers will manipulate prefrontal cortex activity on a trial-to-trial basis using electrical stimulation in non-human primates and optogenetic silencing in mice.
procedureTo test the predictions of Integrated Information Theory (IIT), researchers will apply pairwise phase consistency analyses to neurons and LFPs significantly activated in response to stimuli in the window between 250 ms post-stimulus onset to stimulus offset for non-human primates (NHPs) and between 150 ms post-stimulus onset to stimulus offset for mice, compared to a baseline of 0–250 ms pre-stimulus onset.
procedureWhen decoding content-specific sensory regions, the researchers use recording data from the sensory region associated with the sensory content in the relevant trial, such as pITC (including PL), mITC (including ML) for faces in NHPs, and VISp for visual gratings in mice.
procedureTo enable cross-species comparisons, the study protocol involves non-human primates (NHPs) and mice viewing or listening to supra-threshold visual or auditory stimuli for variable durations in a go-nogo task that controls for report confounds, while recording neuronal responses from visual, auditory, posterior parietal, and/or prefrontal cortical areas using Neuropixels electrodes.
procedureThe researchers will perform control trials in mice using manipulation outside the sensory stimulation epoch and using wild-type mice that do not express channelrhodopsin; for non-human primates, control trials will involve electrical stimulation during central fixation only.
procedureNon-human primates and mice perform a go-nogo behavioral task in which they must discriminate target stimuli from non-target relevant stimuli and from task-irrelevant stimuli.
procedureTo causally test predictions about the timing and location of the neural correlates of consciousness, the study protocol involves manipulating activity in prefrontal cortical regions using electrical stimulation in non-human primates or optogenetic silencing in mice.
procedureTo test the predictions of Global Neuronal Workspace Theory (GNWT), researchers will apply pairwise phase consistency analyses between significantly responsive neurons/LFPs (in the window 250–500 ms post-stimulus onset for NHPs and 150–400 ms post-stimulus onset in mice, compared to the baseline 0–250 ms pre-stimulus onset) and all other LFPs/neurons located in putative GNWT brain areas, based on the theory's prediction that early local sensory response is broadcast during ignition into the global workspace.
procedureTo test functional connectivity predictions, the researchers will calculate spike-LFP pairwise phase consistency for each area pair, comparing consistency at pre-stimulus onset (0–250 ms) with consistency during the stimulus onset window (250–500 ms post-stimulus onset for non-human primates; 150–400 ms post-stimulus onset for mice) and the stimulus duration window.
procedureThe researchers will manipulate prefrontal cortex (PFC) activity from 0–0.5 seconds after stimulus onset in non-human primates and mice for irrelevant stimuli, using a single stimulus duration of 0.5 seconds.
procedureThe researchers will insert probes into the right hemisphere of non-human primates due to a potential right-hemispheric bias for face processing, while probes in mice will be inserted into the left hemisphere to utilize existing surgical and electrophysiological infrastructure.