concept

primed pluripotent stem cells

Also known as: PSCs, primed PSCs, primed human embryonic stem cells

Facts (12)

Sources

Mitochondria and the dynamic control of stem cell homeostasis link.springer.com Springer Apr 16, 2018 12 facts

claimThe conversion of primed pluripotent stem cells (PSCs) to naïve PSCs is facilitated by STAT3-mediated activation of mitochondrial respiration and the induction of oxidative phosphorylation (OXPHOS) genes following the down-regulation of LIN28.

referenceLIN28 regulates stem cell metabolism and the conversion to primed pluripotency, as reported by Zhang et al. (2016).

claimAcetyl-CoA-dependent histone acetylation plays a role in the maintenance of primed pluripotent stem cells (PSCs).

claimNaïve pluripotent stem cells (PSCs) correspond to the pre-implantation stage of embryo development, whereas primed pluripotent stem cells (PSCs) correspond to the post-implantation stage.

claimRecent studies have investigated the functional and molecular differences between naïve and primed pluripotent stem cell (PSC) states to understand the relevance of mitochondrial metabolism for pluripotency.

claimPyruvate dehydrogenase kinases (PDK) 1 and 3 are over-expressed in cancer cells and primed pluripotent stem cells (PSCs), where they inhibit the pyruvate dehydrogenase complex (PDC) and redirect pyruvate away from the mitochondria into lactate.

claimGlutamine is indispensable for the survival of primed pluripotent stem cells (PSCs).

referenceThe metabolome regulates the epigenetic landscape during the transition from naive to primed human embryonic stem cells, as reported by Sperber et al. in 2015.

claimNaïve pluripotent stem cells (PSCs) exhibit higher oxidative phosphorylation (OXPHOS) activity than primed pluripotent stem cells (PSCs), despite being potentially less developmentally mature.

claimPrimed pluripotent stem cells (PSCs) prefer generating mitochondrial acetyl-CoA from glycolysis-derived pyruvate through the action of the pyruvate dehydrogenase complex (PDC).

claimNaïve pluripotent stem cells (PSCs) show higher oxidative phosphorylation (OXPHOS) activity than primed pluripotent stem cells (PSCs), yet naïve PSCs possess a less tubular and non-fused mitochondrial morphology compared to primed PSCs.

claimIn primed pluripotent stem cells (PSCs), alpha-ketoglutarate (aKG) has been found to both accelerate differentiation and support the undifferentiated state, suggesting it exerts opposite effects depending on the cellular context.