procedure
Rastogi et al. developed an enzymatic-based technique for valorizing lignocellulosic biomass into lignin-free polysaccharides using sugarcane bagasse and corn cob as feedstock. The procedure involves: (1) alkali treatment with 2% NaOH for delignification, (2) xylanases enzyme treatment extracted from Aspergillus tubingensis strains, (3) substrate loading at 2% (w/v), (4) enzyme loading at 100–500 U g−1, and (5) incubation at 40 °C, 140 rpm, for 72 hours. The saccharification yield was 81.4%, with greater saccharification observed in the hemicellulose fraction than the cellulose fraction.

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