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Renukaji Wildlife Sanctuary

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Investigation of nutritional and phytochemical properties of wild ... nature.com Nature Dec 9, 2025 19 facts

claimThe study investigated the ethnomedicinal applications, elemental composition, and nutritional content of ten wild medicinal plant species collected from the Renukaji Wildlife Sanctuary in the Sirmaur district of Himachal Pradesh.

procedureResearchers used the UPLC-PDA method to conduct a quantitative analysis of medicinal plants collected from the Renukaji Wildlife Sanctuary in Sirmaur.

measurementIn an analysis of medicinal plants from Renukaji Wildlife Sanctuary, Cassia fistula L. had the highest flavonoid levels at 0.630 ± 0.100, and Berberis lycium Royle had the lowest at 0.343 ± 0.110.

claimThe landscape of the Renukaji Wildlife Sanctuary is characterized by northern boundaries covered with Sal (Shorea robusta) forests, which transition to sub-tropical pine (Pinus roxburghii) forests at higher altitudes.

claimThe medicinal plants studied in the Renukaji Wildlife Sanctuary contain bioactive compounds including saponins, alkaloids, and flavonoids, as well as essential minerals including Na, N, K, P, Zn, Fe, Cu, Mn, Ca, Mg, and S.

accountResearchers conducted a field-based ethnobotanical survey in the Renukaji Wildlife Sanctuary from 2020 to 2023.

measurementThe Renukaji Wildlife Sanctuary in Sirmaur, Himachal Pradesh, covers an area of 4.03 km² and is situated at an altitude ranging from 640 to 950 meters above mean sea level.

claimThe indigenous inhabitants of Renukaji Wildlife Sanctuary in Sirmaur, Himachal Pradesh, utilize locally sourced medicinal plants to treat conditions including diabetes, jaundice, cold, cough, piles, skin disorders, and stomach problems.

claimThe Renukaji Wildlife Sanctuary is located within the catchment area of the Giri River at Dadahu, near Nahan town, in the outer range of the Western Himalayas.

claimThe plant species Achyranthes aspera L. is utilized by the indigenous inhabitants of Renukaji Wildlife Sanctuary for medicinal purposes.

measurementThe leaves of Achyranthes aspera L. collected from the Renukaji Wildlife Sanctuary contain the following concentrations of secondary metabolites: Rutin (1.267 µg/10 mg), Gallic acid (0.183 µg/10 mg), Pro-catechuic acid (0.182 µg/10 mg), P-coumaric acid (0.623 µg/10 mg), and Ferulic acid (0.080 µg/10 mg).

claimThe concentration of Epicatechin in Berberis lycium Royle roots is higher in samples collected from the Renukaji Wildlife Sanctuary, likely due to environmental and ecological factors influencing secondary metabolite synthesis.

measurementRoots of Berberis lycium Royle collected from Renukaji Wildlife Sanctuary contained 4.187 µg/10 mg of Epicatechin and 0.147 µg/10 mg of Gallic acid.

measurementIn an analysis of medicinal plants from Renukaji Wildlife Sanctuary, Aegle marmelos (L.) Correa had the highest saponin content at 1.530 ± 0.100, and Syzygium cumini (L.) Skeels had the lowest at 1.253 ± 0.115.

procedureThe plant collection and identification procedure involved gathering information on local names, growth habits, plant parts used, treated ailments, and methods of use through questionnaires and interactions with local healers, villagers, and farmers in the Renukaji Wildlife Sanctuary between 2020 and 2021.

claimThe ten wild medicinal plant species studied in the Renukaji Wildlife Sanctuary are Achyranthes aspera L., Aegle marmelos (L.) Correa, Azadirachta indica A. Juss., Berberis lycium Royle, Cassia fistula L., Senegalia catechu (L.f.) P.J.H. Hurter & Mabb., Senna tora (L.) Roxb., Syzygium cumini (L.) Skeels, Tinospora cordifolia (Willd.) Hook.f. & Thomson, and Vitex negundo L.

claimResearchers analyzed the medicinal plants Achyranthes aspera L., Berberis lycium Royle, and Vitex negundo L. gathered from the Renukaji Wildlife Sanctuary in Sirmaur for polyphenols and flavonoids using the UPLC-PDA method (Waters Acquity UPLC H-class).

measurementIn an analysis of medicinal plants from Renukaji Wildlife Sanctuary, Aegle marmelos (L.) Correa had the highest alkaloid content at 0.493 ± 0.035, and Azadirachta indica A. Juss. had the lowest at 0.353 ± 0.100.

claimUPLC analysis of the methanolic leaf extract of Achyranthes aspera L. collected from the Renukaji Wildlife Sanctuary in Sirmaur, Himachal Pradesh, confirmed the presence of Rutin, Gallic acid, Protocatechuic acid, P-coumaric acid, and Ferulic acid.