entity

non-human primate

Also known as: NHP

Facts (12)

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Protocol for testing global neuronal workspace and integrated ... journals.plos.org PLOS ONE 12 facts

procedureIn NHP craniotomy surgery, researchers perform up to five small (2 mm) craniotomies within the recording chamber above target cortical sites, using 3D printed parts to hold probes, which are secured to the skull with dental acrylic and a micromanipulator system to seal the site.

claimGlobal Neuronal Workspace Theory (GNWT) requires that decoder performance in the posterior parietal cortex (PPC) after prefrontal cortex (PFC) manipulation is lower in mice and different in non-human primates (NHPs) to be considered a successful test result.

procedureTo test the Global Neuronal Workspace Theory (GNWT) prediction that conscious processing is represented by an ignition signal in the prefrontal cortex (PFC) and posterior parietal cortex (PPC) just after stimulus onset, the protocol assesses whether average decoding performance generalization is above chance level in the stimulus onset window, defined as 250–500 ms after stimulus onset for non-human primates (NHPs) and 150–400 ms after stimulus onset for mice.

procedureTo test the location of conscious content, the researchers will assess average decoding performance in the prefrontal cortex (PFC), posterior parietal cortex (PPC), and content-dependent sensory regions during the stimulus onset window (250–500 ms post-stimulus for NHPs; 150–400 ms post-stimulus for mice) and the stimulus duration window.

procedureFor non-human primate (NHP) subjects, the researchers include only trials occurring during stable task performance, defined as an overall accuracy of greater than 75% per session.

procedurePost-operative care for NHP subjects includes the administration of the antibiotic cefazolin (up to 30 mg/kg IM) and the analgesic agent buprenorphine (0.01–0.02 mg/kg IM, every 8–12 hours for at least the first 48 hours).

claimGlobal Neuronal Workspace Theory (GNWT) predicts that decoding performance in the posterior parietal cortex (PPC) will be affected by prefrontal cortex (PFC) manipulation, with performance expected to be lower for mice and either higher or lower for non-human primates (NHPs), based on the inhibitory effect of the PFC manipulation.

measurementThe study enforces a sample size limitation of 15 recording sessions in each non-human primate (NHP) and, for mice, 4 recordings per area per mouse, with a maximum of 20 mice.

procedureThe researchers will shift the mouse time window earlier than the non-human primate (NHP) window to account for the known faster processing speeds of neuronal signals to the cortex in mice.

procedureThe surgical procedure for securing an implant to a non-human primate (NHP) skull involves drilling 12 threaded holes (2.5 mm diameter) around the planned margin of the implant, securing the implant with ceramic skull screws and acrylic, attaching a head post to the left side of the skull with acrylic, and positioning a recording chamber over the right hemisphere secured with screws and plastic nuts.

procedureIf euthanasia is necessary for NHP subjects due to poor health, it is performed with an overdose of sodium pentobarbital (or equivalent) as approved by a clinical veterinarian and in accordance with the American Veterinary Medical Association Panel on Euthanasia recommendations.

procedureTo test the Global Neuronal Workspace Theory (GNWT) prediction that an ignition signal occurs after stimulus offset, the researchers will assess whether the spike rate in the Prefrontal Cortex (PFC) and Posterior Parietal Cortex (PPC) during the stimulus offset window (250–500 ms after stimulus offset for non-human primates, and 150–400 ms after stimulus offset for mice) is greater than the baseline spike rate (0–250 ms before stimulus onset) using t-tests and Bayesian t-tests.