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microbial cells
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Adaptive laboratory evolution – principles and applications for ... link.springer.com Jul 1, 2013 10 facts
procedureBatch cultivation in shake flasks involves propagating microbial cells in parallel serial cultures where an aliquot of the culture is transferred to a new flask with fresh medium at regular intervals, typically daily, for an additional round of growth.
claimMicrobial cells are advantageous for adaptive laboratory evolution studies because they have simple nutrient requirements, are easily cultivated in the laboratory, and grow rapidly, allowing for several hundred generations to be cultivated within weeks or months.
measurementDNA replication in microbial cells has a high fidelity with a mutation rate of approximately 10^-10 per base pair per replication.
claimThe advent of transcript and next-generation sequencing technologies has enabled recent studies to successfully apply adaptive laboratory evolution to engineer microbial cells for biotechnological applications.
claimGene amplifications contribute to increased gene dosage and subsequently increased fitness in microbial cells.
claimMicrobial cells possess an intrinsic capacity to balance self-preservation (stress protection) and nutritional competence, a concept known as the SPANC balance.
measurementThe specific growth rates of microbial cells used in adaptive laboratory evolution are typically in the range of 0.05 to 1.0 h-1.
claimMicrobial cells, specifically bacteria and yeasts, are utilized in biotechnological tasks including biofuel production, commodity chemical synthesis, and the production of industrial and biopharmaceutical proteins.
claimMicrobial cells have evolved mechanisms to cope with changing environmental conditions, such as fluctuations in temperature, pH, oxygenation, pressure, and nutrient availability, to maintain cellular homeostasis.
claimRecombinant protein production and metabolite co-production in microbial cells are processes coupled to microbial growth.