concept

laboratory evolution

Also known as: laboratory-evolved

Facts (14)

Sources

Adaptive laboratory evolution – principles and applications for ... link.springer.com Springer Jul 1, 2013 14 facts

claimLaboratory evolution can achieve balanced protein expression levels more easily than standard molecular biology techniques.

referenceAraya et al. (2010) performed whole-genome sequencing of a laboratory-evolved yeast strain.

claimDhar R et al. studied the adaptation of Saccharomyces cerevisiae to saline stress through laboratory evolution.

claimStable phenotypes accumulate rapidly during laboratory evolution, though population heterogeneity can arise due to clonal interference, bet hedging, genetic hitchhiking, and fluctuating growth environments.

claimResearchers introduced a heterologous transcriptional regulator from Deinococcus radiodurans into Escherichia coli to select for improved phenotypes, demonstrating that extensive perturbations of regulatory networks can expand possibilities in rapid laboratory evolution towards complex traits.

claimResearchers achieved a drastic increase in n-butanol tolerance in Escherichia coli by combining laboratory evolution with genome shuffling of the evolved clones.

claimThe principles of laboratory evolution experiments are based on the work of scientists including Antonie van Leeuwenhoek, Louis Pasteur, Robert Koch, and Charles Darwin, specifically regarding their discoveries of microorganisms, the germ theory, and the role of natural and artificial selection.

claimLarge-scale transcriptional re-arrangements and gene amplifications are common during laboratory evolution.

claimPredicting the scale of improved phenotype emergence during laboratory evolution is generally difficult, though predictions are possible in specific growth contexts such as carbon source adaptation.

referenceDe Kok et al. (2012) identified new lactate transporter genes in a jen1Δ mutant of Saccharomyces cerevisiae as ADY2 alleles using laboratory evolution, whole-genome resequencing, and transcriptome analysis.

claimHigh mutation rates and mutator strains can emerge naturally during laboratory evolution and contribute to the formation of genetic novelty.

procedureLaboratory evolution experiments can accelerate the selection process by increasing the diversity of targets for selection through the application of chemical mutagens, mutants deficient in DNA repair, or transposon libraries.

referenceY. Deng and S.S. Fong published the paper 'Laboratory evolution and multi-platform genome re-sequencing of the cellulolytic actinobacterium Thermobifida fusca' in the journal Journal of Biological Chemistry in 2011 (Volume 286, pages 39958-39966).

claimTranscriptional changes during laboratory evolution and specialization can lead to trade-offs or cross-benefits in alternative environments by shifting the balance between stress protection and nutritional competence.