procedure
The gradient elution system for UPLC-PDA analysis uses mobile phase A (0.1% formic acid in water) and mobile phase B (0.1% formic acid in ACN) at a flow rate of 0.30 mL/min, starting at 5% B, increasing to 30% B (0.3-9 min), increasing to 70% B, holding at 50% B (9-12 min), and returning to 5% B at 12.2 min.

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