The procedure for determining alkaloid content in plant samples involves combining 5 g of dried leaf extract with 200 mL of 10% acetic acid in ethanol, allowing the mixture to sit for 5 hours, filtering the blend, concentrating the filtrate to one-fourth of its original volume, adding concentrated NH4OH to induce precipitation, washing the precipitate with dilute NH4OH, filtering again, and drying and weighing the resulting residue.

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• Person: Not available Organization: Nature
  Investigation of nutritional and phytochemical properties of wild ...

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• Investigation of nutritional and phytochemical properties of wild ... www.nature.com Nature via serper

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